An Introduction To DNA

An Introduction To DNA DNA (deoxyribonucleic acid) is the genetic material of eve living organisms including some viruses. It is a dimer consists of two strands that immerse upon each other and appear as a double helix that are linked together covalently with each other. Each strand is made up of similar repeating units called nucleotides. Each nucleotide composed of three different moieties,a 2-deoxyribose sugar,a phosphate group and a nitrogenous base. 1.1.1 2-Deoxyribose sugar The 2-deoxyribose sugar, a major structural component of DNA is a cyclic molecule .The sugars are joined together by phosphate groups that form phosphodiester bonds between third and fifth carbon atoms of adjacent sugar rings.The 5†² carbon of deoxyribose sugar is attached to the 3†² carbon of the next, and make a network of 3†² carbon and 5†² carbon.5’end of a DNA molecule is characterized by a free phosphate (P) group and the 3†² end is characterized by a free hydroxyl (OH) group. It lacks an hyd roxyl group at the 2 position as in a ribose therefore a sugar moiety is a 2-deoxyribose. Two free hydroxyl groups are also located on the 5 carbon and 3-carbon of 2-deoxyribose sugar.These hydroxyl groups give a DNA oligomer its designation of 5 and the 3 end(usually accent as â€Å"three prime end† and â€Å"five prime end†). 1.1.2 Sugar-Phosphate backbone The 2-deoxyribose sugar and a phosphate group forms the backbone in the DNA which are highly polar and defines directionality of the molecule. The polar hydrophilic back- bone is surrounded by a core of hydrophobic bases and is important for the stability and structure of DNA. The phosphate groups have a negative charge that gives a concentra- tion of negative charge on the backbone of DNA and also makes DNA,a negatively charge 5 1 Fundamentals molecule. The charge is also neutralised by DNA-binding proteins that contain the pos- itively charged amino acids lysine and arginine, which are attracted to the negatively charged phosphate backbone. See Fig. 1.1. Figure 1.1: DNA backbone 1.1.3 Nucleic acid bases DNA contain four different nitrogenous bases that make monomer of one nucleotide different from other. These bases are adenine (A), thymine (T), cytosine (C), and gua- nine(G). The bases come in two categories pyrimidines and purines. Larger nucleic acids adenine and guanine are members of a class of doubly ringed structures called purines while the smaller nucleic acids cytosine and thymine are members of a class of singly- ringed chemical structures called pyrimidines .A six-membered ring with two-nitrogen molecule formed a pyrimidine structure whereas purine is produced by a nine-membered, ring with four- nitrogen molecule. Each unit of the ring constructing the base is numbered to for specific identification. They are arranged in a particular order along the backbone of DNA to make a long chain of varying sequence that contains the code for proteins.The sequence specifies the exact genet ic instructions required to create a particular organism with its own unique traits. 6 1 Fundamentals 1.1.4 Base Pairing in DNA The nitrogenous bases are responsible to form double-strand of DNA in consequence of weak hydrogen bonds and have specific shapes and hydrogen bond properties. The three hydrogen bonds form between guanine and cytosine and then denoted as G.C or C.G,depending on which is associated with the first strand. Similarly adenine and thymine also bond exclusively by pairing of two hydrogen bonds and then denoted as A.T or T.A. This coupling up of nitrogen bases termed as complementarity.,A hydrogen bond donor need an equivalent hydrogen bond acceptor to form a hydrogen bond in the base across from it. Purines are only complementary with pyrimidines because molecules in pyrimidine-pyrimidine pairings are very far from each other that doesn’t makes the hydrogen bonding to be established. Purine-purine pairing are energetically unfavourable because the molecule s are too close and create an electrostatic repulsion. The only possible pairings are GT and AC. Primary and secondary amine groups or hydroxyl groups are common hydrogen bond donar while carbonyl and tertiary amines are common hydrogen bond acceptor groups. There are two hydrogen bonds between an A:T base pair. One hydrogen bond lie between the 6†² primary amine of adenine and the 4†² carbonyl of thymine. The other hydrogen bond form between the 1†² tertiary amine of adenine and the 2†² secondary amine of thymine. On the other hand,G:C base pair has three hydrogen bonds. One hydrogen bond lie between guanine with its 6†² hydrogen bond accepting carbonyl and cytosine having 4†² hydrogen bond accepting primary amine. The second hydrogen bond also formed between guanine on 1†² secondary amine and cytosine 3†² tertiary amine and the third formed between the 2†² primary amine on guanine and the 2†² carbonyl on cytosine.

Fashion retail concepts of the future Essay Example | Topics and Well Written Essays - 500 words - 1

Fashion retail concepts of the future - Essay Example The essay "Fashion retail concepts of the future" analyzes Fashion retail. In future fashion retail, there will be considerable use of online systems. A website legitimizes the existence of a business. E-commerce performs a physical role in enabling convenient access of products across distant areas. More fashion stores may face the pressure of opening their online versions to facilitate convenience of shopping among customers. In supply chain management, technology will be crucial in enabling efficiency. RFID technology helps track the movement of clothes in the distribution chain. This is possible though the use of tags that help track products from the manufacturing phase to the purchase stage. Fashion is ushering in an age of interactive shopping. Interactive experience means that a client takes charge of one’s shopping time. This involves the use of sensory simulation in testing products before purchase. For instance, the Burberry store in central London avoids the idea o f direct purchases. The store has simulated the website experience in its physical store on Regent Street. A customer, therefore, makes one’s purchases from the sofa while using a swipe machine. Clothing in the store has chips and identification technology that makes it possible for customer to witness images of clothes in catwalk. Mobile applications will be a key feature in fashion retail. Major brands such as SalesGossip, Burberry, Forever21, Shopstyle, and Tommy Hilfiger are investing in various applications.

How the theory of Strategic Human Resource Management can assist Essay

How the theory of Strategic Human Resource Management can assist organisations in achieving and sustaining competitive advantage - Essay Example The paper tells that the increasing complexities and difficulties of management gave rise to the concept of managing at the strategic level. Managing at the strategic level is concerned with the long-term positioning of the organization. With the changes in the work environment, the private sector started recognizing that apart from financial and technological capital, human capital can also provide the competitive advantage. This led to innovation in HR practices or strategic human resources management. Delery and Doty defined strategic HRM practices as those that are â€Å"theoretically or empirically related to overall organizational performance†. Strategic Human Resources Management (SHRM) is about linking people with the organization – integrating HRM strategies into corporate strategies. In other words, SHRM aims to align or integrate decisions about people with the results that the organization aims to achieve. This is done because it is believed that integrating business strategy with HRM can lead to effective management of human resources and thereby enhanced organizational performance. The primary role of HR in the past has been compliance with laws, rules, and regulation. If the HR departments have a future-orientation and if the HR strategies operate as an integral part of the overall business plan, firms can achieve competitive advantage, according to Budhwar and Aryee. SHRM strategies can be used for knowledge creation, storage, and dissemination in organizations. HR practices influence an organization’s social climate which in turn leads to knowledge exchange and better organizational performance. Many researchers and scholars have contributed to strategic HRM with their own models and processes. For instance, Storey developed a model that demonstrates a shift from traditional personnel management to HRM. This model comprises of four key aspects – a constellation of beliefs and assumptions, strategic thrust informing d ecisions about people management, the involvement of line managers and shaping of employment relationship through commitment rather than forceful compliance. According to Storey, since the model has the linkage between employment policies and corporate plan, it can lead to competitive advantage. This model also identifies that strategic approach to HRM can be developed by bringing about changes in various elements of HRM functions such as recruitment and selection, training and development, and the conditions of employment. He suggested changes such as nurturing the workforce rather than monitoring them and management action should be based on the business need rather than following set procedures. Devolution to line managers has been proposed by many scholars as a core aspect of SHRM. SHRM has led to redefining the roles of the top management. Certain functions and issues are too complex for the top management to handle and devolvement of HRM to the line managers can lead to the fa ster response.

ireland Essay -- essays research papers

Ireland Geography, Topography, and a Political Perspective There are four provinces in Ireland: Connacht (western Ireland), Munster (southern Ireland), Leinster (eastern Ireland), and Ulster (Northern Ireland). The Republic of Ireland is comprised of the provinces of Connacht, Munster, and Leinster; the province of Ulster is referred to as Northern Ireland and is under Britain’s jurisdiction. Northern and Southern Ireland are differentiated not only by geographical differences, but also by political and religious views. Approximately 5/6 of the 27,136 square mile island is referred to as the Republic of Ireland and claimed freedom during the year 1922, when it separated from Britain’s rule. This was not an easy transition of political power for the Irish, but rather the beginning of a tumultuous war between Northern Ireland and the Republic. They fought not only because the Republic wanted Britain to relinquish power over Northern Ireland, but also because of a difference in religious values and beliefs (Spencer, 14). Primarily Roman Catholic prior to the mid 16th century, Ireland was influenced by England’s schism from the Roman Catholic Church during the reign of King Henry VIII. Only a small percentage (about 10%) of the Republic of Ireland is Protestant, including Methodist and Presbyterian, however Northern Ireland is predominately Protestant and thus this religious rivalry has played an integral role in the separation of Northern and Southern Ireland and has been the foundation for many political issues and disputes. Interestingly, this religious division is not apparent within the Republic of Ireland because they do not feel threatened by the minority of Protestants politically or religiously (Spencer, 26). Subsequently, religion plays an important part within the Irish culture, as well as its political history. It was not until The Good Friday Peace Agreement, signed in 1998, that the Protestants and Catholics reached a cease-fire and agreed to stop the fighting and vandalism (Spencer, 14) The importance of religion to the Irish culture is exemplified through the role of religious characters throughout literature and film, as apparent in Sheridan’s The Field. Topographically, there are several features unique to Ireland; the bogs are one of the most significant topographical features that exemplify Ireland’s uniqueness. Ireland’s climate is co... ...an.com/field).† One of the more dramatic scenes in the film, where the wealthy American, Bull, and Bull’s son (Tadgh) confront each other concerning the sale of a sentimental piece of land, is set near the Erriff River, which is located at Aasleagh Falls near the village of Leenane. As our cinematic journey progresses, we are taken to Dublin where young Fiona, the main character of The Secret of Roan Inish, had initially resided with her father until he deemed it in her best interest to return to her grandparents fishing village in Ireland’s County Donegal to live. Fiona is intrigued by the view of the Island of Roan Inish which can be seen from the coast line of this western Irish village (The Secret of Roan Inish). On a journey similar to Fiona’s, the two young boys, Ossie and Tito in Into the West also begin their journey in the urban and metropolitan eastern Ireland city of Dublin. The boys, guided by a magical horse named Tir na nOg, journey into t he western regions of Ireland. Our cinematic journey is an excellent means by which to expose students to the various geographical characteristics of Ireland and the significant differences between the eastern and western regions.

In Cold Blood Literary Analysis Essay

In Cold Blood, written by Truman Capote, is a book that encloses the true story of a family, the Clutters, whose lives were brutally ended by the barrel of a 12-gauge shotgun. The killers were 2 men, each with 2 different backgrounds and personalities, each with his own reasons to take part in such a harrowing deed. Capote illustrates the events leading up to the murder in sharp detail and describes its aftermath with such a perspective that one feels that he is right there with the culprits, whose names are Dick Hickock and Perry Smith. They had very critical roles in the murder and how they themselves were caught, and in many ways they were foils for one another. Through Capote’s extensive descriptions of Dick and Perry, and his use of dialogue, imagery, and point-of-view, he makes their individual roles in the story evident and makes clear the fact that they counterbalance each other, with their opposite personalities playing major parts in the Clutter murder case. Dick and Perry’s upbringings were vastly contrasting, and their effects were evident as the men grew and developed. Capote illustrates Perry’s childhood with explicit detail and does not hold back. Perry’s upbringing was marred with violence, tragedy, and misdirection. His mother and father were divorced, and constantly had issues with each other. His mother became an alcoholic, and eventually died upon choking on her own vomit(106). He lived in, by all means, a broken home. His entire family, save for one sister, had suffered in some sort of way. Perry gives the gist of his family’s status with saying, â€Å"Jimmy a suicide. Fern out the window. My mother dead. Been dead eight years. Everybody gone but dad and Barbara. ‘† (Capote 134). Perry’s sister Fern had fallen out of a hotel window to her Khan 2 death, with police finding a bottle of alcohol in her possession. Perry’s other brother shot himself, and Perry’s father walked out of his children’s lives when they were all quite young. Not long after that, Perry was put in an orphanage run by nuns. They would whip him constantly for wetting his bed (Capote 93), which was due to Perry having a weak liver (Capote 210). Because of his experience in the orphanage, Perry gave up on God and religion. When he was still a teenager, he joined the US Army and fought in the Korean War. After he came home, he got into a grisly motorcycle accident, mangling both his legs and stunting his growth. When he recovered, he went to go work with his father up in Alaska, which, after several years, ended in an argument from a biscuit, Perry’s father aiming a gun at him, and all of Perry’s possessions being thrown out of the house. So Perry decided to leave. â€Å"I picked up my guitar and started on down the highway† (136). Through all of his early life, it is evident that Perry had to deal with terrible tragedies, a lack of parenthood, and the fact that he never truly had a true home. He never fully learned compassion, mercy, respect, or the value of human life. None of these values were ever shown to him, nor was he ever required to show them to anyone, and in addition, it made him resent many people in his life. As Dr. Jones, a psychiatrist who studied Perry, concluded: â€Å"His childhood,†¦. was marked by brutality and lack of concern on the part of both parents. He seems to have grown up without direction, without love, and without ever having absorbed any fixed sense of moral values†(297) Perry’s upbringing played a major role in carrying out the murder of the Clutter family, and contrasted sharply with Dick’s early life. Dick Hickock’s upbringing was more structured and fortunate, the opposite of Perry’s. His parents had been there to support him, and he did well in school. When asked about his son’s school life, his father responded by exclaiming,†Basketball! Baseball! Football! Dick was always the star player. A pretty good student, too, with A marks in several subjects† (Capote 158). Dick had a fruitful education, and was even offered a scholarship, to which Dick simply says, â€Å"†¦ but I never attended any of them† (Capote 278). After high school, Dick took several small-time jobs, including working at a Khan 3 motor company, â€Å"when I [Dick] had an automobile wreck with a company car. I was in the hospital several days with extensive head injuries† (Capote 278). The car accident caused his face to be slightly maligned, and, as concluded by Dr. Jones, caused residual brain damage and instability in his personality (Capote 286). Dick’s father also confirmed that Dick had changed after the crash, expressing that â€Å"He just wasn’t the same boy† (Capote 255). After experiencing the crash, many parts of Dick’s life began to go downhill. His marital life twisted woefully, and when all was said and done, he had gone through 2 marriages. He did not have a steady job anymore, and he began to commit petty crimes, such as writing bad checks and stealing. The latter resulted in him being in jail, where he had met Perry, who was behind bars for burglary as well, among other charges. Dick and Perry had both agreed to kill the Clutters, however when the time came, Dick showed signs of doubt and lingered, while Perry had almost no hesitation. This seems to point back to each man’s past, where Perry, who had a rougher childhood, did not seem to think twice about killing the family. His scarred adolescence points to a more cold-hearted, bitter, and lonely person, while Dick’s more favorable youth showed signs of mercy and conscience. Perry and Dick’s relations with other people shaped their own respective character and personalities, including their tendencies towards other people. Dick is a self-assured, commanding and charismatic person who seems to think of himself as quite masculine. He’s had a few girlfriends, and more than one wife as well (Capote 277 – 279). He was a distinct athlete in high school, and was by no means anti-social. He is a very smooth-talker, and does not have any trouble using people to his advantage (Capote 97). His ease with people as well as the large amount of people he had befriended made him more sympathetic towards the Clutters, and, if anything, made him more understanding towards other people. Perry, to contrast, did not have the self-assurance or confidence Dick had. Since he was a child, he essentially had no one, as his family was essentially gone, and he moved from place to place. He had been abused by the nuns as a child (Capote 25), had essentially no friends throughout Khan 4 his short time at school, and was bullied often, in which cases he references the â€Å"Big Yellow Bird† carrying him away form his oppressors (Capote 265). Perry himself once expressed, â€Å"My friends are few; those who really know me fewer still† (Capote 225). He has relations with a small amount of people, and among the people who he’s ever interacted with, Perry has ended up resenting a great many of them. With so much dislike for such a large number of people, Perry has a distinctly negative view of people and society in general. This leads to him essentially having no mercy for the Clutters, rather treating it like it had to be done, and that there was nothing he could do about the murders. Additionally, Perry had been a lonesome individual for most of his life, so as he travels with Dick, he becomes quite attached to him, even after their relationship sours a bit. This seems to point itself out when Perry mentions, in a potential escape plan, â€Å"What about Hickock? All preparations must include him† (Capote 265). If Perry was planning to escape, adding another individual to worry about and the risk of getting caught is much higher. Yet Perry is too attached to simply let Dick go, but Dick in on another floor regretting that he didn’t kill Perry when he had the chance. Both Dick and Perry’s relationships with other people reflect in their personalities and attitudes towards contemporary society. Perry’s passive, dainty nature and Dick’s reckless, aggressive, asserting nature serve to offset each other’s behavior to an extent. Perry does not try to assert himself, but rather conforms to what other people, who he deems ‘larger’ than him, tell him. He is the one to worry about everything, as shown when he is concerned about wearing stockings so that no one will recognize them (Capote 37), if a torn glove is a bad omen (Capote 65), and in addition to the countless times afterward that he’d talked about the authorities tracing the crime back to them, that somehow they did have a witness (Capote 109). Dick is the more daring individual. He frequently acts upon a notion and carefully considers the consequences AFTER the deed has been done. He does tend to be reckless sometimes, as he became when he was writing bad checks, and used his real name in the same city he committed the murder of the Clutters in (Capote 213). With Dick nudging Perry on in whatever scheme they were Khan 5 plotting, and with Perry holding Dick back and thinking it through, they seemed to get both a bold plan along with attention to detail. In the book, Capote gracefully shows how Perry’s actions and tendencies function and respond with Dick’s personality and habits, making it clear that they are foils for one another. Works Cited Capote, Truman. In Cold Blood. Boston, MA: G. K. Hall, 1993. Print.

5 Tips for Improving Your Spanish

Analysis Of The Poem Mummy - 931 Words

A grin spread across the young girls face as she knelt by the waters of the River Laun, watching her reflection ripple and dance around on the light, almost like some sort of beautiful ghost like creature. A will o the wisp possibly, was that what it was called. As she thought she herself, she heard and splash and her head shot up in time to see a fish flopping back into the water, a watery thud resonating across the crystal blue river. Mummy! Did you see that Mummy? It jumped out of the water! the child giggled as she turned her head to look at her mother, almost falling in as she did so. Lynn sweetie. Come away from the bank dear. she heard her mother sigh and a frown spread across the eleven year olds face, before she obediently did as she was told, standing up and walking next to her mother, grinning at the sleeping four year old in the woman s arms. Hello Aylon... she giggled, trying to wake the sleeping girl as she poked the sweet little thing. We could go play if she was awake Mummy, couldn t we? Endralynn asked expectantly looking up at her mother through wide eyes. Not today Lynn. Why don t you go help your father talk to the ferryman? the woman suggested and Lynn immediately grinned, running off down the river bank to try and find her father. Daddy, Daddy! Do you need any help? she shouted as her father came into sight, only to watch as he looked at her and shook his head, before gesturing for her to go back to her mother and sister. Well this isShow MoreRelated An Analysis of Sylvia Plaths Poem, Daddy Essay793 Words   |  4 PagesAn Analysis of Sylvia Plaths Poem, Daddy Sylvia Plaths famous poem Daddy seems to refer quite consistently to her deceased father (and obliquely to her then estranged husband Ted Hughes) by use of many references that can clearly be associated with the background of Otto Plath, emphasizing his German heritage. These include the Polish town where Otto was born, the atrocities of the German Nazis in the Second World War (Dachau, Auschwitz, Belsen), the Luftwaffe, and even the professorialRead MoreThe Importance Of Innocence In Wendy Copes Reading Scheme1245 Words   |  5 Pagesillusion of reality to protect what the individual desires to be true to what is actual. In Wendy Cope’s poem â€Å"Reading Scheme,† Cope writes about an affair more from the perspective of children by using the villanelle form to illustrate the inability of the children to make connections and ultimately argue that innocence is an illusion. In order to understand the illusion of innocence in Wendy Cope’s poem we will first familiarize ourselves on the topic of innocence by looking at two different articlesRead MoreStudy Notes on Out of the Blue by Simon Armitage3230 Words   |  13 PagesAnalysis of the poem Simon Armitages poem Out of the Blue is taken his from 2008 anthology of the same name. According to the books publishers, the poems in the anthology are presented in the form of a respone to  three separate conflicts, all of which have  changed the  world we live  in. Told from the point of view of an English trader working in the North Tower of the World Trade Centre, the poem forms part of the film Out Of The Blue  commissioned by Channel 5 and broadcast five years afterRead MoreThe Bird Image in Yeats Poems2062 Words   |  9 Pagesadaption of the bird image, therefore, echoes the traditional and universal values, which facilitates readers to make sense of his otherwise obscure, complex and mysterious poetry. In light of Yeats’ gyre theory of the universe, a close study of Yeats’ poems respectively written in three stages of the poet’s life demonstrates the bird-soul symbolism in three transformed phases of the poet’s beliefs evolving from i dealism, eternity to reincarnation. 1. Idealism of Early Yeats It is obvious that theRead MoreMental illness and Mental Disorders in Catcher in the Rye by J.D. Salinger2045 Words   |  9 Pageslike-â€Å"It’s ‘If a body meet a body coming through the rye’!† old Phoebe said. â€Å"It’s a poem. By Robert Burns† (Salinger 173). Holden mistook the nature of a â€Å"song†, which actually turns out to be a â€Å"poem†, but something that was completely false. The deeper meaning of this shows that Holden often misses the actual event and is selective when it comes to harsh, new things. â€Å"After I came out of the place where the mummies were, I had to go to the bathroom. I sort of had diarrhea..I didnt mind the diarrheaRead MoreW1 Active Adj14109 Words   |  57 Pageswords in both spoken and written English, based on statistical analysis of the 390 million words contained in the Longman Corpus Network – a group of corpuses or databases of authentic English language. The Longman C ommunication 3000 represents the core of the English language and shows students of English which words are the most important for them to learn and study in order to communicate effectively in both speech and writing. Analysis of the Longman Corpus Network shows that these 3000 most frequent